Folatos y su disponibilidad en alimentos evaluados por cromatografía líquida (hplc). validación y aplicaciones en calida nutricional de alimentos
- Olivares Martínez, Ana Belén
- Bernal Cava, María José
- Ros Berruezo, Gaspar Francisco
- Martínez Graciá, María del Carmen
- Periago Gastón, María Jesús
ISSN: 0213-5434, 1989-1784
Année de publication: 2004
Número: 20
Pages: 59-73
Type: Article
D'autres publications dans: Anales de veterinaria de Murcia
Résumé
In this article it is presented the most precise chromatographic method to determine the different forms of B9 vitamin (folic acid or folacin) in foods, besides their availability (mono or polyglutamates). It is based in high performance liquid chromatographic method (HPLC) which protocol permits the extraction, purification and separation of folic acid and its different derivatives forms. This method has been validated with the standards and certified reference materials and compared with others investigation groups versus microbiological assays.