Effect of glucose on trehalose mobilization in Candida utilis

  1. Arguelles Ordóñez, Juan Carlos 1
  2. Vicente Soler, Maria Jerónima 1
  3. Gacto Fernández, Mariano 1
  1. 1 Universidad de Murcia
    info

    Universidad de Murcia

    Murcia, España

    ROR https://ror.org/03p3aeb86

Revista:
Microbiologia Española

ISSN: 0026-2595

Ano de publicación: 1985

Volume: 38

Número: 1-2

Páxinas: 27-34

Tipo: Artigo

GOOGLE SCHOLAR lock_openAcceso aberto editor

Outras publicacións en: Microbiologia Española

Resumo

Exponentially growing cells oí Candida utilis contain less endogenous trehalose than stationary phase cells. The lower levels of stored trehalose in actively growing cells are due not only to decreased trehalose synthesis but also to increased catabolism of the reserve disaccharide. Glucose appears to be involved in the regulation of the two pathways that determine net trehalose accumulation: this sugar inhibits trehalose synthase activity (EC 2.4.1.15) in vitro and simultaneously activates trehalose hydrolase activity (EC 3.2.1.28)

Referencias bibliográficas

  • CABIB, E., and LELOIR, L. F. 1958. The biosynthesis of trehalose phosphate. J. Biol. Chem., 231, 259-75.
  • ELBEIN, A, D. 1967. Carbohydrate metabolism in streptomycetes. II. Isolation and enzymatic synthesis of trehalose. J. Bacterio!., 94, 1520-24.
  • LILLIE, S. H., and PRINGLE, J. R. 1980. Reserve carbohydrate metabolism in Saccharomyces cerevisiae: responses to nutrient limitation. J. Bacteriol., 143, 1384-94.
  • LLOYD, J. B., and WHELAN, W. J. 1969. An improved method for enzymic determination of glucose. Anal. Biochem., 30, 46770.
  • LONDESBOROUGH, J., and VARIMO, K. 1984. Characterization of two trehalases in baker's yeast. Biochem. J., 219, 511-18.
  • LOWRY, O. H.; ROSEBROUGH, N. J.; FARR, A, L., and RANDALL, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193, 265-75.
  • ORTIZ, C. H.; MAIA, J. C. C; TENAN, N. N.; BRAZPADRAO, G. R.; MATTOON, J. R., and PANEK, A D. 1983. Regulation of yeast trehalase by a monocyclic, cyclic AMP-dependent phosphorylation-dephosphoiylation cascade system. J. BacterioL, 153, 644-51.
  • PANEK, A D. 1962. Synthesis of trehalose in baker's yeast. Arch. Biochem. Biophys., 98, 349-55.
  • PANEK, A D., and MATTOON, J. R. 1977. Regulation of energy metabolism in Saccha romyces cerevisiae. Ach. Biochem. Biophys., 183, 306-16.
  • STEWART, P. R. 1975. Analytical methods for yeasts. In: D. M. PRESCOTT (éd.). Methods in Cell Biology, 12, 111-147. Academic Press, New York.
  • THEVELEIN, J. M. 1984. Regulation of trehalose mobilization in fungi. Microbiol. Rev., 48, 42-59.
  • THEVELEIN, J. M., and JONES, K. A. 1983. Reversibility characteristics of glucose induced trehalase activation associated with the breaking of dormancy in yeast ascospores. Eur. J. Biochem., 136, 583-87.