Rapid differentiation of Alectoris rufa L., 1758 and Alectoris chukar (Gray, 1830) (Galliformes: Phasianidae) by melting curve analysis of a parathyroid hormone gene SNP
ISSN: 1138-3399, 1989-2128
Année de publication: 2014
Número: 36
Pages: 121-127
Type: Article
D'autres publications dans: Anales de biología
Résumé
Current DNA-based methods for identifying introgression levels of closely related species imply post-polymerase chain reaction manipulations that requires additional time and expenses for sample processing. Melting curves analysis is a technique that uses species-specific primers. Here we show this method to be highly accurate for the rapid differentiation of pure individual partridges of Alectoris chukar and A. rufa species, using a SNP of the parathyroid hormone gene in a simple, rapid and efficient way. Seventy eight individuals were analyzed, (A. chukar, n=15; A. rufa, n=63), and no hybrids were detected. This method should find wide use for the management polices based on strict genetic controls for partridge stocks reproduced in farms and used in restocking or other hunting activities.