Rapid differentiation of Alectoris rufa L., 1758 and Alectoris chukar (Gray, 1830) (Galliformes: Phasianidae) by melting curve analysis of a parathyroid hormone gene SNP

  1. Rodríguez-García, María Juliana
  2. Galián Albaladejo, José
Revista:
Anales de biología

ISSN: 1138-3399,  1989-2128

Any de publicació: 2014

Número: 36

Pàgines: 121-127

Tipus: Article

DOI: 10.6018/ANALESBIO.36.19 DIALNET GOOGLE SCHOLAR lock_openDIGITUM editor

Altres publicacions en: Anales de biología

Resum

Current DNA-based methods for identifying introgression levels of closely related species imply post-polymerase chain reaction manipulations that requires additional time and expenses for sample processing. Melting curves analysis is a technique that uses species-specific primers. Here we show this method to be highly accurate for the rapid differentiation of pure individual partridges of Alectoris chukar and A. rufa species, using a SNP of the parathyroid hormone gene in a simple, rapid and efficient way. Seventy eight individuals were analyzed, (A. chukar, n=15; A. rufa, n=63), and no hybrids were detected. This method should find wide use for the management polices based on strict genetic controls for partridge stocks reproduced in farms and used in restocking or other hunting activities.

Fuente de los datos: Dialnet