Estudio in vitro de la citotoxicidad de seis adhesivos de prótesis sobre fibroblastos humanos
- Garcia Guirao, Maria Andrea
- Miguel Ramón Pecci Lloret Director
- Julia Guerrero Gironés Director
Defence university: Universidad de Murcia
Fecha de defensa: 18 December 2020
- Ricardo Elías Oñate Sánchez Chair
- José Antonio Moreno Rodríguez Secretary
- Sergio López García Committee member
Type: Thesis
Abstract
To compare the cytotoxicity of six commercially available denture adhesives on human gingival cells: Corega Sin Sabor, Kukident Pro Protección Dual, Novafix Sin Sabor, FiftyDent, Corega Acción Total and Kukident Protección Dual. Material and methods: Eluates of denture adhesives were brought into contact with human gingival cells. Cell toxicity was assessed for cell viability (MTT assay), cell morphology (immunofluorescence assay), apoptosis/necrosis and reactive oxygen species (Flow cytometry assay). Also, the pH of each sample was determined. Data were performed using one-way analysis of variance (ANOVA) and a Tukey test. Significance was considered when p ? 0.05. Results: All denture adhesives tested led to a reduction in pH, especially Kukident Pro Protección Dual and Kukident Protección Dual (***p<0.001). The cell viability assay showed that Kukident Pro Protección Dual and Kukident Protección Dual decreased cell viability significantly at all dilutions (***p < 0.001). Compared with the control group and the rest of the adhesives Corega Sin Sabor showed a significantly higher cell viability score at all dilutions (***p < 0.001). Kukident Protección Dual and Kukident Pro Protección Dual were responsible for necrosis, and the number of cells was much reduced, with an aberrant morphology and pyknotic nucleus. Finally, Kukident products promoted ROS production in gingival cells (***p < 0.001). Conclusions: The results suggest that denture adhesives containing zinc may inhibit cell viability, stimulate ROS production, provoke aberrant cell morphology, and induce apoptosis and cell death. Keywords: biocompatibility; cytotoxicity; human gingival cells, denture adhesives.