Estudio in vitro de la citotoxicidad de seis adhesivos de prótesis sobre fibroblastos humanos

  1. Garcia Guirao, Maria Andrea
unter der Leitung von:
  1. Miguel Ramón Pecci Lloret Doktorvater
  2. Julia Guerrero Gironés Doktormutter

Universität der Verteidigung: Universidad de Murcia

Fecha de defensa: 18 von Dezember von 2020

Gericht:
  1. Ricardo Elías Oñate Sánchez Präsident
  2. José Antonio Moreno Rodríguez Sekretär/in
  3. Sergio López García Vocal
Fachbereiche:
  1. Dermatología, Estomatología, Radiología y Medicina Física

Art: Dissertation

Zusammenfassung

To compare the cytotoxicity of six commercially available denture adhesives on human gingival cells: Corega Sin Sabor, Kukident Pro Protección Dual, Novafix Sin Sabor, FiftyDent, Corega Acción Total and Kukident Protección Dual. Material and methods: Eluates of denture adhesives were brought into contact with human gingival cells. Cell toxicity was assessed for cell viability (MTT assay), cell morphology (immunofluorescence assay), apoptosis/necrosis and reactive oxygen species (Flow cytometry assay). Also, the pH of each sample was determined. Data were performed using one-way analysis of variance (ANOVA) and a Tukey test. Significance was considered when p ? 0.05. Results: All denture adhesives tested led to a reduction in pH, especially Kukident Pro Protección Dual and Kukident Protección Dual (***p<0.001). The cell viability assay showed that Kukident Pro Protección Dual and Kukident Protección Dual decreased cell viability significantly at all dilutions (***p < 0.001). Compared with the control group and the rest of the adhesives Corega Sin Sabor showed a significantly higher cell viability score at all dilutions (***p < 0.001). Kukident Protección Dual and Kukident Pro Protección Dual were responsible for necrosis, and the number of cells was much reduced, with an aberrant morphology and pyknotic nucleus. Finally, Kukident products promoted ROS production in gingival cells (***p < 0.001). Conclusions: The results suggest that denture adhesives containing zinc may inhibit cell viability, stimulate ROS production, provoke aberrant cell morphology, and induce apoptosis and cell death. Keywords: biocompatibility; cytotoxicity; human gingival cells, denture adhesives.