Micropropagación y análisis bioquímico de mimbrera, castaño y ciruelos cultivados en biorreactores con diferentes concentraciones de sacarosa

Abstract

The effect of sucrose concentration in the culture medium on the development of axillary shoots of three woody species grown in vitro in liquid medium in bioreactors has been investigated, with the aim of achieving their photoautotrophic propagation. The plant material consisted of two clones of Castanea sativa x Castanea crenata, which were grown in a Plantform® temporary immersion system and in a 6-liter continuous immersion system, two local varieties of Prunus domestica and one clone of Salix viminalis, which were grown in RITA® temporary immersion bioreactors. To achieve photoautotrophic propagation, cultures were grown with medium or high light intensity (50 or 150 μmol m-2 s-1) and were supplemented or not with CO2 enriched air. In order to characterize the effect of sucrose and culture conditions on the development of explants, their morphological measurements, total soluble phenolic compounds, antioxidant compounds, photosynthetic pigments, sugar content, rooting capacity and acclimatization were analyzed. It has been possible to grow willow without exogenous sugar and reduce the concentration of conventional sugar in chestnut and plum trees by up to one sixth and one third, respectively.