Dissecting the transcriptional response to elicitors in Vitis vinifera cells

  1. Almagro, L. 2
  2. Carbonell-Bejerano, P. 4
  3. Belchí-Navarro, S. 2
  4. Bru, R. 1
  5. Martínez-Zapater, J.M. 4
  6. Lijavetzky, D. 3
  7. Pedreño, M.A. 2
  1. 1 Universitat d'Alacant
    info

    Universitat d'Alacant

    Alicante, España

    ROR https://ror.org/05t8bcz72

  2. 2 Universidad de Murcia
    info

    Universidad de Murcia

    Murcia, España

    ROR https://ror.org/03p3aeb86

  3. 3 Universidad Nacional de Cuyo
    info

    Universidad Nacional de Cuyo

    Ciudad de Mendoza, Argentina

    ROR https://ror.org/05sn8wf81

  4. 4 Instituto de Ciencias de la Vid y del Vino
    info

    Instituto de Ciencias de la Vid y del Vino

    Logroño, España

    ROR https://ror.org/01rm2sw78

Revista:
PLoS ONE

ISSN: 1932-6203

Año de publicación: 2014

Volumen: 9

Número: 10

Tipo: Artículo

DOI: 10.1371/JOURNAL.PONE.0109777 SCOPUS: 2-s2.0-84907938504 WoS: WOS:000343662500077 GOOGLE SCHOLAR

Otras publicaciones en: PLoS ONE

Resumen

The high effectiveness of cyclic oligosaccharides like cyclodextrins in the production of trans-resveratrol in Vitis vinifera cell cultures is enhanced in the presence of methyl jasmonate. In order to dissect the basis of the interactions among the elicitation responses triggered by these two compounds, a transcriptional analysis of grapevine cell cultures treated with cyclodextrins and methyl jasmonate separately or in combination was carried out. The results showed that the activation of genes encoding enzymes from phenylpropanoid and stilbene biosynthesis induced by cyclodextrins alone was partially enhanced in the presence of methyl jasmonate, which correlated with their effects on trans-resveratrol production. In addition, protein translation and cell cycle regulation were more highly repressed in cells treated with cyclodextrins than in those treated with methyl jasmonate, and this response was enhanced in the combined treatment. Ethylene signalling was activated by all treatments, while jasmonate signalling and salicylic acid conjugation were activated only in the presence of methyl jasmonate and cyclodextrins, respectively. Moreover, the combined treatment resulted in a crosstalk between the signalling cascades activated by cyclodextrins and methyl jasmonate, which, in turn, provoked the activation of additional regulatory pathways involving the up-regulation of MYB15, NAC and WRKY transcription factors, protein kinases and calcium signal transducers. All these results suggest that both elicitors cause an activation of the secondary metabolism in detriment of basic cell processes like the primary metabolism or cell division. Crosstalk between cyclodextrins and methyl jasmonate-induced signalling provokes an intensification of these responses resulting in a greater trans-resveratrol production.