XVII Premio “Ilustre Colegio Oficial de Veterinarios de Madrid” Monitorización de la circulación de Leishmania Infantum en Primates no humanos en cautividad en España

  1. Jesús Barbero Moyano 1
  2. Remigio Martínez Pérez 1
  3. Moisés Gonzálvez Juan 1
  4. Inmaculada Moreno Iruela 1
  5. Adrián Beato Benítez 1
  6. David Cano Terriza 1
  7. Rocío Canales Merino 1
  8. Andrea Ferreiro Prado 1
  9. María Ángeles Risalde Moya 1
  10. Ignacio Antonio García Bocanegra 1
  1. 1 Universidad de Córdoba
    info

    Universidad de Córdoba

    Córdoba, España

    ROR https://ror.org/05yc77b46

Revue:
Anales de la Real Academia de Ciencias Veterinarias

Année de publication: 2023

Volumen: 31

Número: 31

Pages: 19-43

Type: Article

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Résumé

Cases of Leishmania infantum infection have recently been reported in non- human primates (NHPs) in Spain. The aim of this study was to determine exposure and risk factors associated with L. infantum infection in NHPs housed in captivity centers in Spain. Between 2007-2023, sera from 252 NHPs were collected at 15 centers. Indirect immunofluorescence was used to detect the presence of antibodies against L. infantum. In addition, hair samples from 78 individuals were tested for Leishmania kDNA by real-time quantitative PCR. Anti-Leishmania antibodies were detected in 4% (10/252; 95% CI: 1.6-6.4) of the NHPs tested. Twenty-two NHPs were longitudinally sampled: one ring-tailed lemur (Lemur catta) seroconverted and a seropositive Bornean orangutan (Pongo pymaeus pymaeus) increased antibody titers during the study period. L. infantum kDNA was found in 62.8% (49/78; 95% CI: 52.1-73.6) of animals. Phylogenetic analysis revealed high homology between the sequence obtained and strains previously isolated in humans, dogs and wildlife species in Spain. This is the first report of Leishmania kDNA detection in NHP hair samples. The results indicate that hair samples could be a useful, non-invasive method of detection of L. infantum infection in these species. This is also the first large-scale survey of L. infantum conducted in NHPs species in Europe. The main risk factors associated with L. infantum infection were age (≥5 years old) and body size (large). Our results demonstrate widespread circulation of this parasite among NHPs housed in Spain. Control programs should be implemented to minimize the risk of NHP exposure to L. infantum

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