Measuring NLR Oligomerization III: Detection of NLRP3 and NLRC4 Complex by Bioluminescence Resonance Energy Transfer

  1. Martín-Sánchez, Fátima 1
  2. Peñín-Franch, Alejandro 1
  3. Angosto-Bazarra, Diego
  4. Tapia-Abellán, Ana 2
  5. Compan, Vincent
  6. Pelegrín, Pablo
  1. 1 Universidad de Murcia
    info

    Universidad de Murcia

    Murcia, España

    ROR https://ror.org/03p3aeb86

  2. 2 Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca (IMIB)
Libro:
Methods in Molecular Biology

ISSN: 1064-3745,  1940-6029

ISBN: 9781071633496,  9781071633502

Año de publicación: 2023

Páginas: 93-103

Tipo: Capítulo de Libro

DOI: 10.1007/978-1-0716-3350-2_6 GOOGLE SCHOLAR lock_openAcceso abierto editor

Referencias bibliográficas

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  • Pfleger KDG, Seeber RM, Eidne KA (2006) Bioluminescence resonance energy transfer (BRET) for the real-time detection of protein-protein interactions. Nat Protoc 1:337–345
  • Xu Y, Kanauchi A, von Arnim AG, Piston DW, Johnson CH (2003) Bioluminescence resonance energy transfer: monitoring protein-protein interactions in living cells. Methods Enzymol 360:289–301
  • Wu PG, Brand L (1994) Resonance energy transfer: methods and applications. Anal Biochem 218:1–13
  • Siddiqui S, Cong W-N, Daimon CM, Martin B, Maudsley S (2013) BRET biosensor analysis of receptor tyrosine kinase functionality. Front Endocrinol (Lausanne) 4:46
  • Xu Y, Piston DW, Johnson CH (1999) A bioluminescence resonance energy transfer (BRET) system: application to interacting circadian clock proteins. Proc Natl Acad Sci U S A 96:151–156
  • Compan V, Baroja-Mazo A, López-Castejón G, Gomez AI, Martínez CM, Angosto D et al (2012) Cell volume regulation modulates NLRP3 Inflammasome activation. Immunity 37:487–500
  • Tapia-Abellán A, Angosto-Bazarra D, Martínez-Benaclocha H, de Torre-Minguela C, Cerón-Carrasco JP, Pérez-Sánchez H, Arostegui JI, Pelegrín P (2019) MCC950 closes the active conformation of NLRP3 to an inactive state. Nat Chem Biol 15:560–564
  • Tapia-Abellán A, Angosto-Bazarra D, Alarcón-Vila C, Baños MC, Hafner-Bratkovič I, Oliva B, Pelegrín P (2021) Sensing low intracellular potassium by NLRP3 results in a stable open structure that promotes inflammasome activation. Sci Adv 7:eabf4468