Microfluidic Model of the Alternative Vasculature in Neuroblastoma
- Villasante, Aránzazu
- López Martínez, María José
- García Lizarribar, Andrea
- Peng, Xiaofeng
- Samitier, Josep
- Joaquín Roca González (coord.)
- Dolores Ojados González (coord.)
- Juan Suardíaz Muro (coord.)
Editorial: Universidad Politécnica de Cartagena
ISBN: 978-84-17853-76-1
Any de publicació: 2023
Pàgines: 566-569
Congrés: Congreso Anual de la Sociedad Española de Ingeniería Biomédica. CASEIB (41. 2023. Cartagena)
Tipus: Aportació congrés
Resum
Neuroblastoma (NB) is a highly vascularized pediatric tumor arising from undifferentiated neural crest cells early in life, exhibiting both traditional endothelial-cell-driven vasculature and an intriguing alternative vasculature. The alternative vasculature can arise from cancer cells undergoing transdifferentiation into tumor-derived endothelial cells (TEC), a trait associated with drug resistance and tumor relapse. The lack of effective treatments targeting NB vasculature primarily arises from the challenge of establishing predictive in vitro models that faithfully replicate the alternative vasculature phenomenon. In this study, we aim to recreate the intricate vascular system of NB in an in vitro context, encompassing both types of vascularization, by developing a novel neuroblastoma-on-a-chip model. We designed a collagen I/fibrin-based hydrogel with an Emod = 0.87 ± 0.28 kPa, closely mirroring NB's physiological composition and tumor stiffness. This ...