Estudio in vitro de las propiedades antibacterianas de cuatro adhesivos autograbantes en Ortodoncia

Abstract

Introduction and objectives: patients wearing fixed orthodontic appliances are at risk of tooth enamel decalcification, which could be avoided using antimicrobial adhesives for bracket bonding. The aims of this study were: 1) To determine the anti-bacterial effectiveness of four self-etching primers (CPB, CSB, TSEP, iBond) against Streptococcus mutans and Lactobacillus gasseri, the main causes of dental caries; 2) To determine the bacterial adhesion of the above mentioned microorganisms to the tested primers. Materials and methods: inhibitory effects of the primers against S. mutans and L. gasseri were examined using the agar diffusion method with Whatman No1 disks loaded with 15 μl of adhesive, UV polymerized, layered on previously inoculated BHI and MRS plates incubated at 37ºC, 48 h microaerobically. Data were analysed with Kruskal-Wallis (p<0.05) and Mann-Whitney tests, applying the Bonferroni correction (p<0.005). Bacterial adhesion was studied with scanning electron microscopy. Results: only CPB and iBond produced a clear inhibition halo against S. mutans and L. gasseri. iBond was the only tested product to which the bacterias adhere on, mainly S. mutans. Conclusions: Only CPB and iBond produced a clear inhibition halo against S. mutans and L. gasseri. iBond was the only tested product to which the bacterias adhere on, mainly S. mutans.