Tuberculosis caprinaEstudio lesional y su relación con el tipo de reacción diagnóstica

  1. Grincalaityte Puodziukynaite, Ramune 1
  2. Buendía Marín, Antonio 2
  3. Párraga Ros, Ester 2
  1. 1 Universidad de Murcia
    info

    Universidad de Murcia

    Murcia, España

    ROR https://ror.org/03p3aeb86

  2. 2 Departamento de Anatomía y Anatomía Patológica Comparada
Revista:
Anales de veterinaria de Murcia

ISSN: 0213-5434,  1989-1784

Ano de publicación: 2022

Número: 36

Páxinas: 83-95

Tipo: Artigo

DOI: 10.6018/ANALESVET.542391 DIALNET GOOGLE SCHOLAR lock_openDIGITUM editor

Outras publicacións en: Anales de veterinaria de Murcia

Resumo

Some regions of Spain have control and eradication programs to fight against goat tuberculosis. These official programs are based on a type of diagnosis that relays on cellular immunity (Intra dermal tuberculin test and IFN-γ release assay), however, there is a humoral type of diagnosis (serology) that could be useful as an ancillary test for the detection of animals with a predominant type of humoral response. Those goats are related to a more advanced phase of the disease and they can spread the bacteria in an easier way causing a persistent infection on some farms. Since these tests are based on the immunopathological process suffered by the animal, it is of our interest to understand better the regulation of the immune response at a systemic and local level. In this study, 23 goats were taken, 10 of which were positive only to cellular immunity-biased tests and the other 13 only positive to humoral-biased tests. From the macroscopic visualization of the lesions, samples were taken to perform 3 different studies: the histopathological one where microscopic lesions were classified as closed or opened. For the etiological study, the Ziehl Neelsen stain was carried out, and finally, for the study of the different lymphocyte families separately, immunohistochemical staining was performed. The objective was to find the existing relationships between the type of diagnostic test and the different studied parameters. The results showed that there was indeed a significant association between a cell-type diagnosis, with closed-type lesions, on the other hand, the association between a humoral-type diagnosis with open-type lesions (more infective ability), with a more significant presence of B lymphocytes as well as regulatory T lymphocytes in the lesion, a reason that meets our hypothesis that these cells are found in a higher number in more advanced stages with chronic inflammation processes.

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Fonte de datos: Dialnet