Evaluación de un método electroforético para el subfraccionamiento de las partículas plasmáticas de lipoproteínas de baja densidad

Resumen

Since NCEP ATP III accepted the small and dense LDL particle as an emergent risk factor of cardiovascular disease, the methods to calculate LDL subfractions have increased. The present report attempts to evaluate the usefulness of a polyacrylamide gel electrophoresis system (LipoprintTM) to separate LDL in our population. 194 blood samples were collected from subjects between 15�94 years old (49% men). Imprecision and lipid parameter study population ranges by sex, and the correlations between them were calculated. Imprecision study results were acceptable. When correlating the average diameter of particle LDL with other lipid markers, we observed an inverse association with triglyceride concentration and Apo B100, and a direct association with HDL-cholesterol. We found differences between sex in triglyceride and LDL-cholesterol levels (greater in men) and HDL-cholesterol and average diameter of LDL particles (greater in women). When comparing the average LDL particle diameter with the lipid parameters, we found that it is inversely associated with the triglyceride and Apo B100 concentration, and directly with HDL-cholesterol, which is associated with a greater cardiovascular risk. We believe that LipoprintTM system is useful for the measurement of the concentration and average diameter of LDL particles, due to its simplicity and speed of results. Nevertheless, studies are needed that can associate the results obtained to the clinical parameters that are used in the evaluation of cardiovascular risk.