Eficacia del dodecil sulfato de sodio para la inactivación de micoplasmas en calostro caprino
- Paterna, A.
- Amores, J.
- Gómez-Martín, Á.
- Corrales, J. C.
- Prats-van der Ham, M.
- Tatay-Dualde, J.
- Contreras, A.
- de la Fe, C.
- Sánchez López, A.
ISSN: 0213-5434, 1989-1784
Year of publication: 2014
Issue: 30
Pages: 77-84
Type: Article
More publications in: Anales de veterinaria de Murcia
Abstract
In small ruminant herds chronically affected with contagious agalactia, replacement animals acquire the infection by suckling the milk and colostrum of infected dams that show no symptoms of the disease. As an alterative to traditional heat treatments such as pasteurisation, this study examines the effect of different concentrations of sodium dodecyl sulfate (SDS) on the viability of Mycoplasma agalactiae and Mycoplasma mycoides subsp. capri in goat colostrum. After the treatment and before incubating contaminated colostrum samples, SDS was removed or not from the samples to assess the effect of prolonged treatment during the incubation period. Ten minutes of treatment with 0.1% SDS were ineffective against both species of mycoplasma. When SDS was used at 1%, no growth was observed when the detergent was present during the incubation period yet viable colonies appeared when SDS was removed just before this period. Our findings indicate that the duration of colostrum SDS treatment is critical for its inactivation effect on these mycoplasmas.