Clonación, expresión, caracterización y modelado de esterasas putativas de Corynebacterium glutamicum ATTC 13032

Abstract

Cloning, expression, characterization and modeling of putatives esterases from Corynebacterium glutamicum ATCC 13032 The aim of this work was to clone, express, purifiy and characterize some genes from Corynebacterium glutamicum ATCC13032, identified as enzymes of the superfamily of esterases/lipases. Among all cloned genes, only the protein encoded by the gene Cg-2528 could be studied. The protein was purified and characterized. The optimum activity was observed at 40ºC and pH 8. The enzyme showed a high activity in presence of organic solvents, such as DMSO or methanol. P-nitrophenyl ester and naphthyl ester derivatives were found to be better substrates than S-methyl tiobutanoate. The catalytic triad was predicted by amino acid sequence alignment and the structure modeling was performed using esterase (2OGS) form Geobacillus stearothermophilus as template.