Evaluación de diferentes estrategias de conservación de semen caprino para el diagnóstico microbiológico de Mycoplasma mycoides subsp. capri
- Pozzoli, M.
- Corrales, J.C.
- Prats-Van Der Ham, M.
- Tatay-Dualde, J.
- De la Fe, C.
- Sánchez, A.
- Contreras, A.
- Gómez-Martín, A.
- M.ª Jesús Alcalde Aldea (coord.)
- Ceferina Vieira Aller (coord.)
- Juan José García García (coord.)
- Valentín Pérez Pérez (coord.)
- Raúl Bodas Rodríguez (coord.)
- Jesse Barandika (coord.)
Editorial: Ediciones Universidad de Salamanca ; Universidad de Salamanca
ISBN: 978-84-9012-793-3
Ano de publicación: 2017
Páxinas: 455-460
Congreso: Sociedad Española de Ovinotecnia y Caprinotecnia (SEOC). Jornadas (42. 2017. Salamanca)
Tipo: Achega congreso
Resumo
Four methods to preserve the goat buck ejaculates used for contagious agalactiadiagnosis were tested. Four aliquots of diluted semen were prepared and contaminatedwith Mycoplasma mycoides subsp. capri (Mmc). Three of them were respectively maintained1) at room temperature, 2) refrigerated (4ºC) or 3) at freezing temperature (-20ºC) withglycerol during 24 h; the forth aliquot was used to submerge a commercial sterile swaband was subsequently conserved at refrigeration temperature, all them during 24 hours.A quantitative or a quantitative analysis of the bacterial viability was later conducted.Culture of aliquots preserved at room temperature and refrigeration showed a higher Mmcconcentration in comparison with the aliquot maintained at freezing temperature (p=0.001and p=0.002). Despite it, results demonstrated that Mmc survived at infective concentrations at -20ºC suggesting the validity of this preservation method for microbiological diagnosticafter 24 hours. Results also showed the validity of refrigerated swabs maintained at 4ºCfor culturing Mmc. Further studies are necessaries to test the suitability of these methodsunder other conditions considering other bacterium concentrations or other mycoplasmaspecies.