La selección espermática en el útero de la cerda está influenciada por la calidad de los espermatozoidesAnálisis del reflujo

Resumen

The aim was to examine the way in which spermatozoa are selected by the uterus after artificial insemination. In total, 25 sows were post-cervical inseminated (1500x106 spz/25ml) using 3 different sperm doses: 1) HIGH motility: 50% of the sperm not stained and with high motility (>70%) + 50% of the sperm stained (with Hoechst 33342) with high motility (>70%); 2) MEDIUM motility: 50% of the sperm not stained with high motility (>70%) + 50% of the sperm stained with medium motility (35-70%); 3) LOW motility: 50% of the sperm not stained with high motility (>70%) + 50% of the sperm stained with low motility (<35%). Backflow was collected at different times after insemination (0-15, 16-30 and 31-60 min) and % of stained sperm was determined using fluorescence microscope (UV). The results showed that during the first moments after insemination (0-15 min) the uterus performs an indiscriminate removal (measure in the backflow) of sperm regardless of their motility (HIGH: 48.81; MEDIUM: 55.29; LOW: 55.45%). During 16-30 and 31-60 min intervals the majority of sperm in backflow were stained in MEDIUM (73.78 and 84.27%, respectively) and LOW (83.57 and 89.85%, respectively) groups but not in the HIGH one (54.47 and 59.37%). The uterus sperm selection is not specific during the first moments after insemination, but later the uterus selects those sperm with better motility, discarding those less competitive in the backflow.