Evaluación del cultivo y de la pcr para el diagnóstico de cabras portadoras del grupo mycoplasma mycoides en el conducto auditivo externo

  1. C. de la Fe 1
  2. J.C. Corrales 1
  3. J. Amores 1
  4. A. Gómez Martín 1
  5. A. Sánchez 1
  6. A. Contreras 1
  1. 1 Universidad de Murcia
    info

    Universidad de Murcia

    Murcia, España

    ROR https://ror.org/03p3aeb86

Book:
XXXIII Jornadas Científicas y XII Internacionales de la Sociedad Española de Ovinotecnica y Caprinotenia: Almería, 24-27 de septiembre de 2008 : producción ovina y caprina, nº XXXIII SEOC

Publisher: Consejería de Agricultura y Pesca ; Junta de Andalucía

ISBN: 978-84-8474-246-3

Year of publication: 2008

Pages: 302-306

Congress: Sociedad Española de Ovinotecnia y Caprinotecnia (SEOC). Jornadas (33. 2008. Almería)

Type: Conference paper

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Abstract

A comparative study of the use of culture and polymerase chainreaction (PCR) to detect asymptomatic carriers of Mycoplasma mycoidescluster from ear swabs was carried out. During a 1 year-period, a total of 283ear swabs were collected from Murciano-Granadina goats. 261 samples werecollected from 3 herds previously diagnosed as infected by Mycoplasma spp.and 22 ear swabs were collected in a free-contagious agalactia (CA) herd andused as negative control. PCR was positive in 235 samples for M. mycoidescluster. MmmLC was identified in all positive samples. A total of 113 sampleswere positive by culture, and all those culture-positive samples, except 2 ofthem, were PCR positive too. Results obtained confirmed the high sensibility ofPCR respect to culture to realise the direct detection of ear carriers ofMycoplasma spp. These results also confirm the high number of auricularcarriers existing in CA infected herd.

Data source: Dialnet