Resistencia a macrólidos en Mycoplasma capricolum subsp. capricolummecanismos moleculares y umbral de sensibilidad

  1. Tatay-Dualde, J. 1
  2. Prats-Van der Ham, M. 1
  3. Gómez-Martín, A. 1
  4. Corrales, J.C. 1
  5. Contreras, A. 1
  6. Sánchez, A. 1
  7. García-Galán, A 1
  8. De la Fe, C. 1
  1. 1 Universidad de Murcia
    info

    Universidad de Murcia

    Murcia, España

    ROR https://ror.org/03p3aeb86

Libro:
XLIII Congreso Nacional y XIX Congreso Internacional de la Sociedad Española de Ovinotecnia y Caprinotecnia (SEOC)
  1. María Jesús Alcalde Aldea (coord.)
  2. Begoña Panea Doblado (coord.)
  3. Valentín Pérez Pérez (coord.)
  4. Raúl Bodas Rodríguez (coord.)
  5. Francisco Saura Armelles (coord.)
  6. Juan José Ramos Antón (coord.)

Editorial: Sociedad Española de Ovinotecnia y Caprinotecnia SEOC

ISBN: 978-84-697-5289-0

Ano de publicación: 2018

Páxinas: 419-423

Congreso: Sociedad Española de Ovinotecnia y Caprinotecnia (SEOC). Jornadas (43. 2018. Zaragoza)

Tipo: Achega congreso

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Resumo

Mycoplasma capricolum subsp. capricolum is one of the causative agents of contagious agalactia, and antimicrobial therapy is the most commonly applied measure to treat outbreaks of this disease. Macrolides bind specifically to nucleotides at domains II and V of the 23S rRNA. Furthermore, rplD and rplV genes encode ribosomal proteins L4 and L22, which are also implicated in the macrolide binding site. The aim of this work was to study the relationship between mutations in these genes and the acquisition of macrolide resistance in M. capricolum subsp. capricolum. For this purpose 14 field isolates were studied. This study demonstrates the appearance of DNA point mutations at the 23S rRNA encoding genes (A2059G and A2062C) and rplV gene (Ala89Asp) in association to high minimum inhibitory concentration values. Moreover, these mutations enable us to provide an interpretative breakpoint of antimicrobial resistance for Mcc at MIC 0.8 μg/ml.

Fonte de datos: Dialnet