Análisis mediante citometría de flujo de la respuesta de los espermatozoides de verraco a diferentes medios de incubación

  1. Matas Parra, Carmen
Supervised by:
  1. Emilio Martínez García Director
  2. Juan María Vázquez Rojas Director

Defence university: Universidad de Murcia

Year of defence: 1997

Committee:
  1. Emilio Espinosa Velazquez Chair
  2. Jordi Roca Aleu Secretary
  3. Agustín Josa Serrano Committee member
  4. Antonio Ramírez Medina Committee member
  5. Anselmo Gracia Molina Committee member
Department:
  1. Animal Medicine and Surgery

Type: Thesis

Teseo: 60018 DIALNET lock_openDIGITUM editor

Abstract

The purpose of the present study was to evaluate the sperm capacitation and acrosome reaction of spermatozoa washed in Percoll and unwashed undergo in an in vitro incubation system with progesterone or cumulus-oocyte and the penetration rate in homologous oocytes matured in vitro. The evaluation was done with lectin (PNA, SJA and ECA) and flow cytometry. The results shown that progesterone had a protective effect on sperm vitality and the highest percentages of acrosome reaction were obtained between 45-90 minutes of incubation. The penetration rate was similar between washed and unwashed spermatozoa.