Interacciones homólogas y heterólogas in vitro de gametos porcinos, bovinos y humanos y sus aplicaciones en el estudio de la fecundación

Resum

The interaction between gametes is crucial to fertilization. The zona pellucida (ZP) is responsible to block of polyspermy, but in vitro these functions are not efficient. The polyspermy is frequently in bovine and porcine in vitro fecundation. Besides the heterologous interaction between spermatozoa-oocyte had been described. The aims were study the block of polyspermy to improve the output of IVF and research the heterologous interactions between human spermatozoa and porcine oocyte. The results show that there is hardening of bovine and porcine ZP previously at fertilization, in vivo and using DTSP or bovine oviductal fluid. When these oocytes are used in IVF improve the monospermy and the output. In heterologus interactions the human spermatozoa could bind to porcine ZP and it triggers the acrosome reaction, but not penetration in ZP-free oocyte was observed. In ICSI the oocyte activation and