Optimización del sistema de fecundación in vitro en la especie porcinacondiciones de maduración y cocultivo en gametos

  1. Almiñana Brines, Carmen
Supervised by:
  1. Emilio Martínez García Director
  2. María Antonia Gil Corbalán Director

Defence university: Universidad de Murcia

Fecha de defensa: 18 February 2008

Committee:
  1. Emilio Eduardo Espínola Chair
  2. José Néstor Caamaño Gualdoni Secretary
  3. Xiomara Lucas Arjona Committee member
  4. Carlos José Robayo Committee member
  5. Lydia Gil Huerta Committee member
Department:
  1. Animal Medicine and Surgery

Type: Thesis

Teseo: 141379 DIALNET lock_openDIGITUM editor

Abstract

The present study was conducted in an attempt to optimize porcine in vitro fertilization system. For this purpose, the influence of maturation and gamete coculture conditions were studied. The coincubation time may be reduced to 10 min to increase the efficiency of fertilization depending on the sperm:oocytes ratio. The needs of boar spermatozoa for IVF, in terms of additives to IVF medium and coincubation times vary among boars. The use of coincubation time as brief as 2 min is long enough to obtain good fertilization rates similar to those achieved from current long term exposure times in IVF. A straw IVF system in combination with a 10 min coincubation increased monospermic penetration and the quality of blastocysts compared with the microdrop-IVF system. The addition of 5 nM of 9- cis retinoic acid in the IVM medium increased blastocyst formation rate, suggesting that RA may play an important role during IVM.