Influencia de diferentes condiciones de cocultivo sobre la fecundación y la producción in vitro de embriones porcinos

  1. Gil Corbalán, María Antonia
Supervised by:
  1. Juan María Vázquez Rojas Director
  2. Emilio Martínez García Director

Defence university: Universidad de Murcia

Fecha de defensa: 04 April 2007

Committee:
  1. Emilio Eduardo Espínola Chair
  2. Inmaculada Rodríguez Artiles Secretary
  3. Agustín Josa Serrano Committee member
  4. Jordi Roca Aleu Committee member
  5. Pedro J. García Herradón Committee member
Department:
  1. Animal Medicine and Surgery

Type: Thesis

Teseo: 82571 DIALNET lock_openDIGITUM editor

Abstract

To decrease the high incidence of polyspermy penetration of porcine oocytes fertilized in vitro (IVF), the aim of this study was to evaluate: 1) the effect of three volume of co-incubation medium (2, 1 and 0.1 ml) and three number of oocytes (50, 30 and 15), inseminated with 6 x l05 sperm/ml (first experience) and 2000:1 spermatozoa:oocyte (second experience); 2) the effect of cumulus cells during IVF of oocytes inseminated with different espermatozoa:oocytes rates (2000: 1, 3000: 1, 4000: 1, 6000: 1 and 8000: 1), in 0.1 ml of volume of IVF medium, with 30 oocytes. Denuded matured oocytes inseminated with 2000 spermatozoa:oocyte were the control group; and 3) the effect of short-times that oocytes are exposed to the sperm during IVF (10, 30 and 60 min) on the efficiency of pig IVF.