Efectos biológicos de cuatro cementos definitivos usados en implantología sobre los fibroblastos gingivales humanos

Abstract

To determinate the biological effects of four cements; RelyxUnicem 2 (RU), Panavia V5 (PV5), Multilink Hibryd Abutment (MHA) and SoloCem (SC) on human gingival fibroblasts. Materials and Methods: Human gingival fibroblasts (HGF) were exposed to different eluates of resin-based cements. The cytotoxic effect and cell migration were assessed using MTT and wound-healing assays. The attachment and the cell morphology were analyzed by Scanning Electron Microscopy (SEM). The levels of intracellular reactive oxygen species (ROS) produced by the eluates of the different cements were determined. Data from results were performed using a one-way analysis of variance (ANOVA) and Turkey&apos;s test (p< 0.05). Results: MHA did not affect cell viability in the first 24h or 72h at any dilution, and it showed significant differences in comparison to the control (**p<0.01) at 48h undiluted and 1:2 dilution. Extracts of SC reduces the viability of HGFs at any time undiluted with significant differences in comparison to the control (***p<0.0001).Cell migration was affected by the undiluted SC group at all period-times compared to the control (***p<0.001). Contrarily, at all period-times and all dilutions studied, cell migration rates in the MHA group were similar to that of the control group. SEM showed very few cells in SC group. Conclusions: MHA eluates showed a better cell migration, greater cell attachment and higher cell viability than those obtained with the other cements. SC group showed worse results in all parameters. Keywords: implant abutment, cytotoxicity, periimplantitis, human gingival fibroblasts, resin-based cement.