Caracterización de biomateriales pre y post aplicación en un modelo in vivo mediante uso de tomografía computerizada

  1. Adrián Hurtado Bermúdez 1
  2. Nuria García Carrillo 2
  3. Miguel Garcés-Villalá 3
  4. Félix de Carlos- Villafranca 4
  5. Francisco Martínez Martínez 5
  6. María del Carmen González Escudero 1
  1. 1 Universidad Católica San Antonio
    info

    Universidad Católica San Antonio

    Murcia, España

    ROR https://ror.org/05b1rsv17

  2. 2 Universidad de Murcia
    info

    Universidad de Murcia

    Murcia, España

    ROR https://ror.org/03p3aeb86

  3. 3 Fundación Corazón de Jesús. San Juan Argentina
  4. 4 Universidad de Oviedo
    info

    Universidad de Oviedo

    Oviedo, España

    ROR https://ror.org/006gksa02

  5. 5 Hospital Universitario Virgen de la Arrixaca. Murcia
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Journal:
Labor dental clínica: Avances clínicos en odontoestomatología

ISSN: 1888-4040

Year of publication: 2021

Volume: 22

Issue: 2

Pages: 10-25

Type: Article

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More publications in: Labor dental clínica: Avances clínicos en odontoestomatología

Abstract

Objective To establish a method that allows us to characterize pre and post application biomaterials, based on the information provided by micro-computed tomo graphy (micro-CT) and bone density measured in Hounsfield Units (HU). Methodology As a biomaterial, 48 dog teeth were used (Premolars P2, P3, P4 and the first molar M1) that were crus hed with Smart Dentin Grinder, 24 of them were sent for micro-CT analysis and 24 samples were la ter implanted. in the alveoli of 6 Beagle dogs. Biop sies were taken three months after implantation and analyzed with the MicroCT. The methodology used was based on a descriptive statistic of the bone density values measured in HU obtained from the creation of volumes of interest (VOIs) and predefi ned 3D iso-contours from the images obtained after performing micro-CT of the biopsies of the crushed tooth (dentin grinder) and its subsequent analysis with the medical imaging data examiner AMIDE. 1 Graduado en Odontología. Universidad Católica de Murcia. Murcia 2 Veterinaria. Universidad de Murcia. Murcia 3 Doctor en Odontología. Investigador Fundación Corazón de Jesús. San Juan Argentina. 4 Profesor Titular de Ortodoncia. Facultad de Medicina . Universidad de Oviedo. Oviedo 5 Servicio de Ortopedia y Traumatología. Hospital Universitario Virgen de la Arrixaca. Murcia 6 Profesora cirugía bucal. Universidad Católica de Murcia Results The micro-CT allows to establish the characteristics of the biomaterials by studying the HU. There is a marked predominance of bone type D3 density (350-850 HU) in the sample with a mean percenta ge of the three values obtained of 96.14%. There is an absence of bone-type density D2 and D1 in 2 of the regions studied, presenting its maximum value at 805.86 HU and 802.67 HU respectively, with a minimum percentage of bone-type density D2 in the region of interest comprised in the ellipsoid 1 (0.08%). We have found the same in biopsies where the presence of D3 bone density (350-850 HU) is prominent in the analysis of the total volume of the selected region of interest comprised in the cylinder with dimensions of 180x180x180 mm by 61%. Conclusions Micro-CT could be considered a technique of great value in the characterization based on the HU of biomaterials, both before and after implantation in an in vivo model. The described method allows us to evaluate the radiological density of both the bioma terial and the biopsies, being necessary the analysis of several parameters of the tissue microarchitecture beyond the HU to establish a reliable ANALYSIS of the quality of the tissue to be evaluated. The results of the micro-CT analysis showed similar densities in UH, both in the pre-implantation samples and in the post-implantation biopsies, similar to bone density D2-D3

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