El desarrollo prenatal del estómago de cabra

Abstract

The aim of this work was to study the goat´s stomach during its prenatal life. A total of 140 goat fetuses at various stages of development from early embryonic stages to birth were used for this research study. The specimens were classified into five groups according to their most revealing histological features: group I (1-25% de gestación); group II (25-35% gestación); group III (35-50% gestación); group IV (50-75% de gestación) y group V (75-100% gestación). The embyos were weighed and measured. Gestational age of each was estimated using as criteria body length, considered as the distance between the highest point of the head and the base of the tail (crown-rump length). Subsequently, the specimens were immersed in 10% formalin. The epithelial layer, lamina propria and submucosa, tunica muscularis and serosa of rumen, reticulum, omasum and abomasum of the goat stomach were analyzed using goat macroscopic and histological analysis by light microscopy and structural analysis by scanning electron microscopy. Also were performed immunohistochemical analysis to detect the presence of neuroendocrine cells (synaptophysin and enolase no neuronal), glial cells (glial fibrillary acidic protein and vimentin) and peptidergic sympathetic and parasympathetic innervations markers (vasoactive intestinal peptide and neuropeptide Y). Finally, histological studies were performed in the compartment wall and each of the tissue layers of said wall constituents to adjust their growth subsequently mathematical models.