Characterization of Ovine Cervical Mucus and Implementation of a Sperm–Mucus Penetration Test Using Fluorescently Labeled Sperm

  1. Abril-Parreño, Laura
  2. Weldon, Kaitlyn
  3. Fair, Sean
Book:
Methods in Molecular Biology

ISSN: 1064-3745 1940-6029

ISBN: 9781071644058 9781071644065

Year of publication: 2025

Pages: 699-708

Type: Book chapter

DOI: 10.1007/978-1-0716-4406-5_48 GOOGLE SCHOLAR lock_openOpen access editor

Bibliographic References

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  • Fair S, Hanrahan JP, Donovan A, Duffy P, O'Meara CM, Lonergan P, Evans AC (2007) Hormonal relationships during the periovulatory period among ewe breeds known to differ in fertility after cervical artificial insemination with frozen thawed semen. Anim Reprod Sci 97(3–4):284–294. https://doi.org/10.1016/j.anireprosci.2006.02.006
  • Abril-Parreno L, Wilkinson H, Krogenaes A, Morgan J, Gallagher ME, Reid C, Druart X, Fair S, Saldova R (2022) Identification and characterization of O-linked glycans in cervical mucus as biomarkers of sperm transport: A novel sheep model. Glycobiology 32(1):23–35. https://doi.org/10.1093/glycob/cwab085
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  • Richardson L, Hanrahan JP, Tharmalingam T, Carrington S, Lonergan P, Evans ACO, Fair S (2019) Cervical mucus sialic acid content determines the progression of thawed ram sperm through the cervix. Reproduction 157:259. https://doi.org/10.1530/REP-18-0547
  • Schimpf U, Caldas-Silveira E, Katchan L, Vigier-Carriere C, Lantier I, Nachmann G, Gidlof S, Jonasson AF, Bjorndahl L, Trombotto S, Druart X, Crouzier T (2022) Topical reinforcement of the cervical mucus barrier to sperm. Sci Transl Med 14(673):eabm2417. https://doi.org/10.1126/scitranslmed.abm2417
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  • O’Hara L, Hanrahan JP, Richardson L, Donovan A, Fair S, Evans AC, Lonergan P (2010) Effect of storage duration, storage temperature, and diluent on the viability and fertility of fresh ram sperm. Theriogenology 73(4):541–549. https://doi.org/10.1016/j.theriogenology.2009.10.009
  • Martinez-Rodriguez C, Alvarez M, Lopez-Uruena E, Gomes-Alves S, Anel-Lopez L, Chamorro CA, Anel L, de Paz P (2015) Ram spermatozoa migrating through artificial mucus in vitro have reduced mitochondrial membrane potential but retain their viability. Reprod Fertil Dev 27(5):852–864. https://doi.org/10.1071/RD13377
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  • Parrish JJ, Susko-Parrish J, Winer MA, First NL (1988) Capacitation of bovine sperm by heparin. Biol Reprod 38(5):1171–1180. https://doi.org/10.1095/biolreprod38.5.1171
  • World Health O (2021) WHO laboratory manual for the examination and processing of human semen, 6th edn. World Health Organization, Geneva