Eficacia de la Cúrcuma Longa (Zingiberacea) vs. Clorhexidina al 0.2% contra bacterias periodontalesestudio in vitro
- Carpio González, Ana María de las Mercedes
- Fabio Camacho Alonso Director
- Massimo Corigliano Director
Defence university: Universidad de Murcia
Fecha de defensa: 07 November 2024
- Francisco José Gómez García Chair
- Francisco Molina Miñano Secretary
- Berta García Mira Committee member
Type: Thesis
Abstract
In recent years, interest in natural antimicrobial agents has been aroused due to high antimicrobial resistance. Cúrcuma Longa (Turmeric), a perennial plant that belongs to the Zingiberaceae family, has generated interest in the scientific community due to its antimicrobial and anti-inflammatory properties. Objective: To determine the antibacterial efficacy of Curcuma extracts at different concentrations vs. 0.2% Chlorhexidine with periodontal strains in vitro: (Porphyromonas gingivalis ATTC ® 33277, Tanneretta forsythia ATTC 43037, Treponema denticola ATTC ®35405, Peptostreptococcus micros ATTC ®33270 and Aggregatibacter actinomycetemcomitans ®ATTC 3338). Methodology: An exploratory, analytical, and longitudinal experimental study was designed. Different types of extracts (ethanolic, methanolic and aqueous) were prepared from Cúrcuma from India and Cúrcuma grown in the Dominican Republic. Curcumas solutions were prepared at different concentrations (100, 50, 25, 12.50, 6.25, 3.125, 1.562, 0.780, 0.390, 0.195 mg/ml) from each one. Five repetitions were performed with each group for a total sample of 185. To evaluate the antibacterial efficacy of Cúrcuma and 0.2% Chlorhexidine, disk diffusion method was performed and modified using nutritional culture media and requirements for each strain according to the Global Bioresource Center (ATTC). The effectiveness of Cúrcumas extracts and 0.2% Chlorhexidine was determined by measuring the efficacy halo in mm. Results: It was observed that Curcumas extracts had efficacy only against Peptostreptococcus micros at a minimum concentration of 50 mg/ml in the (ethanolic and methanolic) extracts and at 100 mg/ml in the (aqueous), with an average efficacy halo of 7mm, while 0.2% Chlorhexidine showed greater efficacy in all strains with an average efficacy halo of 11.8 mm. Conclusion: Extracts from the rhizomes of Cúrcuma have antibacterial efficacy against Peptostreptococcus micros at a minimum concentration of 50mg/ml. The effectiveness of 0.2% Chlorhexidine gel is higher than those of Cúrcuma and equality hypothesis between both products is rejected with a p value = 0.00 < 0.05. However, Chlorhexidine continues to be the topical antiseptic agent used in periodontal therapy with greater antibacterial efficacy.