Potencialidad de la Fibroína de Seda como Scaffold para reparación y regeneración pulpar

Abstract

Silk fibroin is a natural protein made by B. Mori insect knowing too as silk worm. It is a material which presents healing and regenerative properties, so it is an optimal biomaterial which could be used in pulp regeneration or reparation. On the other hand, mesenchymal stem cells (MSCs) are undifferentiated cells that present clonogenic capacity, self-renewal and multipotent differentiation properties, MSCs can differenciate into multiple celulars lineages. They could be found in some different locations inside the mouth. In this study we used stem cells from deciduous teeth (SHEDs). The aim of this study was to evaluate the biocompatibility of silk fibroin with stem cells from deciduous teeth. Pulp extraction was made from deciduous teeth without pulpal involvement reasons and it was enzymatically and mechanically scatters. The recount and the estimation of the cellular viability were executed, after that SHEDs sowing was done. Afterwards a silk fibroin scaffold was prepared, where 5000 cells were sown. Cell proliferation was analyzed by Alamar Blue and morphology was analyzed by scanning electron microscopy (SEM). Cell survival and cell morphology were studied, the results showed that the fibroin scaffolds are biocompatible to SHEDs because they keeps their morphology, sticks on it easily and were able to proliferate on it. This results "in vitro" support silk fibroin scaffold used in tissue structure engineering with stem cells from deciduous teeth. New in vivo studies are needed to confirm it possible use in pulpotomy and regenerative endodontics.